NIRS in Research. Near-infrared spectroscopy (NIRS) is a relatively new technology that has shown utility for many different non-invasive protocols for physiologic experimentation. Essentially, NIRS technology monitors changes in capillary oxygenation values by measuring the intensity of light (600-1000nm) after it passes through biological tissue, (i.e. skeletal muscle). The amount of light that is absorbed by a tissue depends mainly on the amount of oxygen that is bound to the chromophores, hemoglobin and myoglobin, underneath the sensor. Therefore, by measuring dynamic changes in the amount of light that passes through a tissue, researchers are able to get an idea of oxygen consumption within the tissue underneath the sensor.
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